肱骨骨不连的手术治疗

目的探讨肱骨骨不连的手术治疗方法及疗效。方法1998年12月～2005年5月共收治肱骨骨不连患者25例,均为肱骨骨折内固定术后发生骨不连,其中3例并发骨髓炎,6例合并不同程度肱骨骨缺损,骨缺损长度为3～6cm。骨不连病程8个月～5年。15例行吻合血管游离腓骨移植,10例采用加压交锁髓内针进行肱骨固定并辅以自体骨植骨。结果术后25例均得到随访,时间6个月～6年2个月。吻合血管游离腓骨移植组中移植的腓骨段均与肱骨干形成骨性愈合,平均骨性愈合时间为3．1个月；交锁髓内针组平均骨愈合时间为3．8个月。按Crates和Whittle肩肘关节功能评价标准,腓骨移植组：优9例,良4例,差2例；交锁髓内针组：优5例,良3例,差2例。结论应用加压交锁髓内针辅以自体骨移植对硬化性肱骨骨不连是一种有效的外科治疗方法；对合并骨髓炎、大段骨缺损及严重骨质疏松的肱骨骨不连,采用吻合血管游离腓骨移植可一期进行修复与重建。     

928株革兰阴性杆菌耐药性监测

目的：了解青岛地区临床分离的重要革兰阴性杆菌的耐药情况以指导合理用药。方法：2001年青岛3家医院临床分离的5种革兰阴性杆菌用Kirby-Bauer法进行药敏试验。结果：5种病原菌均占3家医院革兰阴性杆菌分离率的前5位，大肠埃希菌对氟喹诺酮类药物的耐药率达60％－87％，细菌对亚胺培南均呈高度敏感，但铜绿假单胞菌耐亚胺培南株占25．8％，头孢他啶的抗菌活性是三代头孢菌素中最高的；复方酶抑制剂表现出良好的抗菌活性。结论：细菌耐药问题仍是目前临床的严重问题，地域性的耐药性监测是必要的。     

醒脑静注射液对脑缺血再灌注小鼠记忆功能的保护作用

目的：观察醒脑静（XNJI）对脑缺血再灌注后小鼠记忆功能的保护作用。方法：采用暂时性阻断两侧颈总动脉的方法制备小鼠脑缺血再灌注损伤的模型。测试其被动回避能力，脑内抗氧化酶活性及小鼠断头喘气时间，结果：XNJI可延长脑缺血再灌注小鼠的避暗潜伏期，减少错误次数，并提高谷胱甘肽过氧化物酶（GSH-Px)的活性。延长小鼠断头喘气时间。结论：XNJI可明显减轻脑缺血再灌注导致的小鼠学习记忆功能障碍状态。其主要机制可能与抗氧化作用有关。     

HIS系统安全与规划管理

本文结合三级乙等医院近10年的管理经验,提供-个网络安全管理的整体解决方案.     

Matrix Metalloproteinase-2 and Tissue Inhibitor of Metalloproteinase-1 in the Retinal Pigment Epithelium and Interphotoreceptor Matrix: Vectorial Secretion and Regulation

Matrix metalloproteinases (MMPs) and the tissue inhibitors of metalloproteinases (TIMPs) play an essential role in both normal and pathological extracellular matrix degradation, and a TIMP has been associated with at least one type of retinal degeneration. We have studied expression of MMP-2 and TIMP-1 by zymography, immunocytochemistry, and immunoblotting in the retinal pigment epithelium (RPE) from normal, aged and diseased retinas. MMPs and TIMPs were found in the rat RPE, interphotoreceptor matrix (IPM), and in media conditioned by human and rat RPE in culture. In other polarized cells, MMPs and TIMP-2 are secreted vectorially towards the basal lamina. In the RPE, however, MMP-2 and TIMP-1 were secreted preferentially from the apical surface, the surface bordering the IPM. These findings provide new evidence that MMPs and TIMPs could play a role in the turnover of IPM components.Cell homogenates and conditioned media from RPE isolated from mutant Royal College of Surgeons (RCS) rats with inherited retinal dystrophy had similar amounts of MMP-2 and TIMP-1 as those from congenic control rats. The secretion of MMP-2 and TIMP-1 from RPE cell cultures isolated from young and aged human donors varied widely. However, with increasing cell passage number, secretion of MMPs and TIMPs from human RPE increased dramatically. Also, growing human RPE on bovine corneal endothelial cell-generated extracellular matrix instead of plastic reduced the secretion of both MMPs and TIMPs. These data suggest that the integrity of Bruch's membrane may serve to regulate RPE functions in MMP and TIMP secretion and that extracellular matrices contain signals that regulate MMP and TIMP synthesis and/or secretion by the RPE.     

两脚和四脚快速腭扩张器疗效的比较研究

目的:本研究比较了两脚和四脚腭快速扩张器在打开腭中缝和牙齿扩张的效果.方法:本研究包括30例患者,随机分配在两脚扩张器组和四脚扩张器组,在扩张器戴入前,扩弓结束后和扩张器拆除时,拍摄标准咬合片和采取上颌模型,并对测量数据进行统计分析.结果:研究发现两组间的磨牙尖宽度、磨牙龈宽度、尖牙尖宽度、尖牙龈宽度和中切牙间裂隙间没有统计学差异;在牙弓的周长和腭中缝打开上略有差异.结论:两脚扩张器和四脚扩张器对腭中缝和牙列的作用相同.     

Complete structure of eclosion hormone of Manduca sexta

The locations of the three disulfide bonds of eclosion hormone (EH) isolated from Manduca sexta were assigned by sequence analysis of thermolysin fragments and by comparison of a key heterodimeric fragment to regiospecifically synthesized parallel and antiparallel isomers. We elucidated the complete structure of Manduca EH as a 62-residue peptide which has three disulfide bonds between Cys¹⁴-Cys³⁸, Cys¹⁸-Cys³⁴, and Cys²¹-Cys⁴⁹.     

测定大黄中总蒽醌含量的分光光度法改进

[目的]改进大黄药材中总蒽醌的含量测定方法。[方法]1,8-二羟基蒽醌与碱显色后,2h内隔时扫描光谱图;对照品和样品同样处理后绘制标准曲线。[结果]确定了最大吸收波长,消除了测定过程中样品湿热降解带来的误差。[结论]通过方法学考察,证明此方法精密准确、回收率高,可以作为大黄中总蒽醌的含量测定方法。     

局部植入BMP对去势大鼠股骨近端生物力学强度及髓腔面积变化的影响

目的探讨去势大鼠股骨近段植入牛骨形成蛋白(bone morphorgeneic protein,BMP)后其骨生物力学强度及髓腔面积的变化。方法随机选取6月龄wistar雌性大鼠22只,摘除卵巢制作绝经后骨质疏松模型成功后,同一只动物双侧肢体对照,试验侧股骨颈植入牛骨形成蛋白及纤维蛋白(FS)复合物,对照侧植入纤维蛋白,术后4周、8周处死取材,测量股骨近端生物力学强度及髓腔面积和截面面积。结果4周后,试验侧与对照侧股骨近端骨生物力学强度和髓腔面积无明显差异;8周后,试验侧股骨近端生物力学强度较对照侧增高;髓腔面积较对照侧有较明显的减小(P<0.05)。结论股骨近端局部植入BMP可提高去势大鼠股骨近端局部的生物力学强度和减小近端髓腔面积和截面积,其可能减少骨质疏松性髋部骨折置换后假体的松动,成为骨质疏松性骨折假体置换中新的辅助治疗方法。     

棘球蚴内容物导致肺损害的研究

目的 :观察棘球蚴囊内容物攻击兔所至肺功能和形态的损害。建立囊型包虫肺栓塞的动物模型。方法 :取囊型包虫内容物 ,分离出囊砂 ,与囊液配成 5 %的悬浊液。 2 1只家兔 ,分 3组 :Ⅰ :生理盐水组 ,Ⅱ :澄清囊液组 ,Ⅲ :囊砂悬浊液组。每只家兔都置入股动脉导管及股静脉导管。依照上述分组按 2mL/kg体重分别自静脉导管注射生理盐水、澄清囊液或含囊砂的悬浊液。于注射后 ( 5、30、6 0min)动态监测MAP、血气指标及血清血管活性物质(ET - 1、TXB2 、6 -keto -PGF1α)水平的变化。指标测定后 ,行肺部ECT检查。最后取出动物肺脏行光镜病检。结果 :Ⅲ组和Ⅱ组在注射后均出现MAP、血气指标的明显降低 (P <0 .0 5 )和血清TXB2 、6 -keto -PGF1α的升高 (P <0 .0 5 ) ,尤以Ⅲ组最明显 (P <0 .0 5 )。各组动物血清ET - 1水平在注射前后无明显变化 (P >0 .0 1)。ECT显示Ⅲ组动物肺部放射性缺损 ,Ⅱ组动物肺部放射性减弱。Ⅲ组肺脏病检见头节广泛栓塞于肺小动脉、微小动脉中 ,肺脏呈“急性呼吸窘迫综合症样”改变。Ⅱ组肺组织出现淤血、水肿及炎细胞浸润。结论 :Ⅲ组动物基本能够模拟出囊型包虫病肺栓塞的临床表现。囊液中有形成分在肺损害中起着重要的作用。TXB2 、6 -keto -PGF1α参与了肺损伤病理过程。